This is a list of the characters in the film Do the Right Thing and the actors who play them.
- Spike Lee as Mookie, a young black man working in Sal's Famous Pizza
- Danny Aiello as Sal, a surly Italian man who owns the pizzeria
- Steve Park as Sonny, a Korean grocery store owner across the street from Sal's
- Ossie Davis as Da Mayor, an older black man who some call the town drunk
- Ruby Dee as Mother Sister, an older black woman who observes the neighborhood goings-ons from the window of her brownstone and despises Da Mayor
- Richard Edson as Vito, one of Sal's sons and a friend of Mookie's
- Giancarlo Esposito as Buggin' Out, an excitable friend of Mookie's who "wants some brothers" on Sal's wall of fame
- Bill Nunn as Radio Raheem, a towering young black man who always carries around a huge boom box blasting only Public Enemy's "Fight the Power"
- John Turturro as Pino, another one of Sal's sons. He is not happy about being one of the last Italians in the neighborhood, nor about his brother's interracial friendship
- Rosie Perez as Tina, Mookie's girlfriend
- Paul Benjamin as ML
- Frankie Faison as Coconut Sid
- Robin Harris as Sweet Dick Willie
- Miguel Sandoval as Officer Mark Ponte, a policeman
- Rick Aiello as Officer Gary Long, a white policeman
- Joie Lee as Jade, Mookie's sister
- Samuel L. Jackson as Senor Love Daddy, the local DJ
- Roger Guenveur Smith as Smiley, a young, mentally impaired man who tries to sell pictures of Malcolm X and Martin Luther King, Jr.
- Steve White as Ahmad
- Martin Lawrence as Cee
- Leonard L. Thomas as Punchy
- Christa Rivers as Ella
- Luis Antonio Ramos as Stevie
- John Savage as Clifton
- Frank Vincent as Charlie
- Richard Parnell Habersham as Eddie
- Ginny Yang as Kim, Sonny's wife